Genotoxicity of Dioxin and its Effect on the Immune Response of Goats Vaccinated with Brucella Melitensis Vaccine

The few cytogenetic studies performed in both humans and animals exposed in vivo or in vitro to dioxin reported conflicting data with or without chromosome damage. Therefore the aim of this work was to fulfill the genotoxicity of dioxin and its effect on immune response of sheep vaccinated with Brucella melitensis Rev.1 vaccine. A total number of twenty female goats were divided into four groups, each group consisted of 5 animals. The first group kept as control till the end of experimental period after 3 weeks post-treated. The second group was vaccinated with the Brucella Rev 1 vaccine. The third group was given an oral dose of 4 ml of stock standard solution of dioxin for 3 successive days. The fourth group was vaccinated with the Brucella Rev 1 vaccine and then given after that the dose of dioxin for each animal for 3 successive days. Blood samples were collected for detection of micronucleus, chromosome aberrations and Brucella antibodies titer. Both cytogenetic tests gave clear indications of high levels of chromosome damage in the dioxin treated group and dioxin vaccinated group compared with the control. Serological tests revealed decreased level of antibodies titer by both Tube agglutination test (TAT) and Mercaptoethanol test (MET) in vaccinated animals plus dioxin. In conclusion, dioxin may induce chromosome damage and lower the immune response of goats vaccinated with Rev.1 vaccine. The percentage of micronuclei and chromosomal aberrations decreased after vaccination with Rev.1 vaccine plus dioxin compared with dioxin alone. [Researcher. 2010;2(1):1-7]. (ISSN: 1553-9865).